PLANT PHYSIOLOGY (BIO 3024) MDH LAB RESULTS

Results from the MDH Lab for the Spring 2018 semester are provided here (updated 4-4-2018).

 

1.  MS-Excel File containing the spreadsheet for protein concentration and MDH enzyme assays [CLICK HERE].  This link includes results that should be included in tabular form for your report.  Note that all results should not be listed in the table.  Only those results you feel are pertinent should be included.

 

2.  Photograph of gel from SDS polyacrylamide electrophoresis of Jilin (Gel #1) and mutant (Gel #2) cell fractionation extracts as well as Fern (Gel #3) and Ivy (Gel #4) cell fractionation extracts.  Here are the lane designations, which should be used in the description of each gel:

 

Lane Number Cell Fraction
1 Standard
2 Crude
3 Chloroplasts
4 Crude Mitochondria 
5 Washed Mitochondria 
6 Cytosol
7 EMPTY
8 EMPTY
9 EMPTY
10 EMPTY

 

3.  Photograph of the Precision Plus Protein Standard for estimating protein size [CLICK HERE]

 

4.  Photographs from Class - showing different steps throughout the experiment.

 

Picture #1 Picture #11 Picture #21 Picture #31 Picture #41
Picture #2 Picture #12 Picture #22 Picture #32 Picture #42
Picture #3 Picture #13 Picture #23 Picture #33 Picture #43
Picture #4 Picture #14 Picture #24 Picture #34 Picture #44
Picture #5 Picture #15 Picture #25 Picture #35 Video #1
Picture #6 Picture #16 Picture #26 Picture #36 Video #2
Picture #7 Picture #17 Picture #27 Picture #37 Video #3
Picture #8 Picture #18 Picture #28 Picture #38 Video #4
Picture #9 Picture #19 Picture #29 Picture #39  
Picture #10 Picture #20 Picture #30 Picture #40  

 

 

As agreed by the class on Monday, April 2, 2018, this lab report may be concise and done in groups (or individually).  Your lab report is due at the beginning of class on Wednesday, April 11, 2018.

 

 

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